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Team:Harvard/fences/results
From 2010.igem.org
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| - | <div><b> | + | <div><b>barnase growth control in <i>E. Coli</i></b> <a href="http://openwetware.org/images/2/2b/M4-growth_curves-10-4-10.jpg" style="font-size:12px">click to enlarge</a></div><hr/> |
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<img src="http://openwetware.org/images/2/2b/M4-growth_curves-10-4-10.jpg" width="600px" border=0> | <img src="http://openwetware.org/images/2/2b/M4-growth_curves-10-4-10.jpg" width="600px" border=0> | ||
Revision as of 23:06, 24 October 2010
results
barnase as growth control agent
This contruct works by expressing background levels of Barstar with Barnase inducible such that it will overwhelm Barstar when induced. Higher levels of Barnase expression resulted in lower rates of growth in the bacteria, affirming the principle of Barnase-based growth control for the genetic fence, and confirming the results from Berkeley 2008. We characterized the growth repression of Barnase under a range of arabinose inducer concentrations.
barnase growth control in E. Coli click to enlarge
