Team:EPF Lausanne/Project parts

From 2010.igem.org

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(BioBricks)
(BioBricks)
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[http://partsregistry.org/Part:BBa_K320006 *C3 + S + Immu]
[http://partsregistry.org/Part:BBa_K320006 *C3 + S + Immu]
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[http://partsregistry.org/Part:BBa_K320007 *C3 + S + P25,]
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[http://partsregistry.org/Part:BBa_K320007 *C3 + S + P25]
[http://partsregistry.org/Part:BBa_K320008 *C3 + S + Immu + ori + Kan]
[http://partsregistry.org/Part:BBa_K320008 *C3 + S + Immu + ori + Kan]

Revision as of 22:16, 24 October 2010



caption

BioBricks

All our parts are in the pSB1C3 backbone vector (abbreviate C3). When you click on the part's name, you are redirected to the partsregistry corresponding page.

First the legend:

  • Ori = Origin of replication for Asaia and E.Coli
  • S = Strong Promoter
  • W = Weak Promoter
  • Immu = immunotoxin
  • P25 = Pfs25 protein
  • P28 = Pfs28 protein
  • Kan = Resistance to Kanamycin
  • Tet = Resistance to Tetracycline

We made basic parts to build the complexest one.

[http://partsregistry.org/Part:BBa_K320000 *C3 + Ori] We made this plasmid because we needed an origin of replication that works in Asaia. After experiences we conclued that it also work with E.Coli

[http://partsregistry.org/Part:BBa_K320001 *C3 + Immu] The immunotoxine is the main part of the project, we obtain it by syntetisation. For a better unerstanding, go there.

[http://partsregistry.org/Part:BBa_K320002 *C3 + S] We made a new strong promoter permitting use to test teh expression of the immunotoxine. We called it strong because we used the constitutive sequence which should give the biggets level of expression.

[http://partsregistry.org/Part:BBa_K320010 *C3 + W] This promoter has been made to have a second level of expression for our proteins, which is lower than the strong one.

We made the following BioBricks from the aboves :

Building the biobricks...

[http://partsregistry.org/Part:BBa_K320003 *C3 + Ori + Tet]

[http://partsregistry.org/Part:BBa_K320004 *C3 + Ori + Kan]

[http://partsregistry.org/Part:BBa_K320005 *C3 + S + CFP]

[http://partsregistry.org/Part:BBa_K320006 *C3 + S + Immu]

[http://partsregistry.org/Part:BBa_K320007 *C3 + S + P25]

[http://partsregistry.org/Part:BBa_K320008 *C3 + S + Immu + ori + Kan]

[http://partsregistry.org/Part:BBa_K320009 *C3 + S + P28 + Ori + Tet]

[http://partsregistry.org/Part:BBa_K320011 *C3 + Ori + Amp]

[http://partsregistry.org/Part:BBa_K320012 *C3 + W + Immu]

[http://partsregistry.org/Part:BBa_K320013 *C3 + W + P25]







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