Team:Harvard/vectors/vectors
From 2010.igem.org
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- | <td style="vertical-align:top"><p style="padding:10px"><br/>The expression series vectors contain a promoter preceding the multiple cloning site such that the inserted construct can be easily expressed through activation of the contained promoter. We modified the vectors pORE E3 and pORE E4. Both vectors contain the ENTCUP2 promoter. pORE E3 confers plant resistance to glufosinate | + | <td style="vertical-align:top"><p style="padding:10px"><br/>The expression series vectors contain a promoter preceding the multiple cloning site such that the inserted construct can be easily expressed through activation of the contained promoter. We modified the vectors pORE E3 and pORE E4. Both vectors contain the ENTCUP2 promoter. pORE E3 confers plant resistance to glufosinate, and pORE E4 confers plant resistance to kanamycin</p></td> |
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Latest revision as of 21:37, 24 October 2010
![](https://static.igem.org/mediawiki/2010/f/fd/Harvard2010vectorbannercrop.png)
meet the vectors
Our vectors are a modification of the pORE agrobacterium vector system developed by Coutu, ''et al'', at Agriculture and Agrofood Canada, which we obtained from The Arabidopsis Information Resource (TAIR). This vector system is designed for agrobacterium mediated plant transformation, and is a binary system with the vector containing the site for foreign constructs and the appropriate flanking regions to be recognized for introduction to the plant. The virulence genes are present on a separate vector contained in the agrobacteria, separate from the TDNA.
To modify the pORE series binary vectors to fit the BioBrick standard, we used a combination of PCR mutagenesis and digestion strategies to replace the vectors' existing multiple cloning site with the BioBrick multiple cloning site. We worked with two of each of the open, reporter, and expression series vectors, creating a total of six BioBrick vectors suitable for agrobacterium mediated plant transformation.
open series click to enlarge ![]() |
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reporter series click to enlarge ![]() |
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expression series click to enlarge ![]() |
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