From 2010.igem.org
(Difference between revisions)
|
|
| Line 105: |
Line 105: |
| | </tr> | | </tr> |
| | </table><p><br /> | | </table><p><br /> |
| | + | |
| | + | <a name="test3"></a><p class="header"><b>Test #3</b></a><p> |
| | + | <table border=0 margin="0" width="600px" padding:0 margin:0> |
| | + | <tr> |
| | + | <td> |
| | + | <img src="https://static.igem.org/mediawiki/2010/5/59/Uhohtest3.jpg"> |
| | + | </td> |
| | + | <td> |
| | + | <ul> |
| | + | <li>We ligated RBS-C0051 to Chris Anderson's Promoter Screening Plasmid.</li> |
| | + | <li>And we have obtained red colonies! We know that the transcription initiation is somewhere in the BBa_C0051 region.</li> |
| | + | </ul> |
| | + | </td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td> |
| | + | <img src="https://static.igem.org/mediawiki/2010/7/7f/Redcolonies.jpg"> |
| | + | </td> |
| | + | </tr> |
| | + | </table> |
| | | | |
| | <p> | | <p> |
Revision as of 21:02, 24 October 2010
|
|
|
|
|
 |
- When we ligated RBS-C0051 to RBS-RFP together, we noticed that there were RED colonies.
- There is no promoter present yet. How this happened was a mystery that we had to look into.
|
 |
|
- First of all, we sequenced the DNA of the red colonies. The sequence analysis showed that we indeed had the correct sequence.
- We decided to run a number of tests to see which part of the sequence exhibited promoter-like behavior.
- Unwanted transcription may be due to the nature of the construct.
- Promoter-like sequence may be present anywhere in the BBa_C0051 or BBa_E1010 (RFP) biobrick, including the LVA and bar code.
|
|
|
|
- We ligated RBS-RFP to RBS-RFP to see if transcription occurred.
- The construct exhibited a white phenotype. Thus, we concluded that transcription didn't start in any part of the RFP
|
|
|
- We ligated RBS-RFP to Chris Anderson's Promoter Screening Plasmid.
- We also obtained white colonies.
|
|
|
|
- We ligated RBS-C0051 to Chris Anderson's Promoter Screening Plasmid.
- And we have obtained red colonies! We know that the transcription initiation is somewhere in the BBa_C0051 region.
|
|
|
|
|
|
|
|
|
|
 |
|---|
|
We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!
We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)
|
|
|
"
