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Team:Yale/Our Project
From 2010.igem.org
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<h3><b>What would it take to... | <h3><b>What would it take to... | ||
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| - | Build a circuit using microbially catalyzed metal deposition?</b><h3/><br/> | + | Build a circuit using microbially catalyzed metal sulfide deposition?</b><h3/><br/> |
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| + | Designing H2S Production | ||
| + | Assaying for H2S Production | ||
| + | Growing Bacteria In Copper Medium | ||
| + | Correlating Bacterial Growth to Copper Deposition | ||
| + | Localizing Copper Deposition | ||
| + | Modeling the Construction of Metal Sulfides | ||
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| + | |||
| + | <h1>Project</h1> | ||
In an effort to achieve our goal, we have designed a bacterial model using synthetic biology. A gene encoding the protein Thisulfate Reductase has been inserted Our initial research indicated that several observations have been made of natural mechanisms for metal deposition and precipitation in a family of microorganisms called Sulfate Reducing Bacteria (SRB). These SRB comprise a class of chemolithotrophic microbes that couple anaerobic electron transport to ATP synthesis using sulfate as the terminal electron acceptor. Importantly, as a consequence of their metabolism, SRB produce hydrogen sulfide: a gas that can be used to reduce metals in solution. <br/> | In an effort to achieve our goal, we have designed a bacterial model using synthetic biology. A gene encoding the protein Thisulfate Reductase has been inserted Our initial research indicated that several observations have been made of natural mechanisms for metal deposition and precipitation in a family of microorganisms called Sulfate Reducing Bacteria (SRB). These SRB comprise a class of chemolithotrophic microbes that couple anaerobic electron transport to ATP synthesis using sulfate as the terminal electron acceptor. Importantly, as a consequence of their metabolism, SRB produce hydrogen sulfide: a gas that can be used to reduce metals in solution. <br/> | ||
Revision as of 19:42, 24 October 2010
our project
Project Overview
Welcome to
Yale-iGEM 2010!
In our inaugural year of iGEM competition, we have designed a system to harness biology to construct conductive circuits.
By enabling E. coli to affect local redox chemistry, we can use bacteria to catalyze metal deposition. When this activity is controlled spatially and temporally, this method could be used to construct circuit elements in a specified geometry. This would allow the manufacture of electrical components under biological conditions.
