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Team:EPF Lausanne/Project parts
From 2010.igem.org
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The BB we have for the moment are: | The BB we have for the moment are: | ||
| - | *C3 + | + | *C3 + Ori |
| - | *C3 + | + | *C3 + Immu |
| - | *C3 + | + | *C3 + S |
| - | *C3 + | + | *C3 + Ori + Kan |
| - | *C3 + | + | *C3 + Ori + Tet |
| - | *C3 + | + | *C3 + S + CFP |
| + | *C3 + S + Immuno | ||
| + | *C3 + S + P25 | ||
| + | *C3 + S + immuno + ori + Kan | ||
| + | *C3 + S + P28 + Ori + Tet | ||
| + | *C3 + Ori + Amp | ||
| + | *C3 + W | ||
| + | *C3 + W + Immun | ||
| + | *C3 + W + P25 | ||
| + | |||
We are actually working on insert the others 2 proteins we want to express (p25, p28) on C3 and trying to finally ligate the BioBricks in the BaseVector containing the origin of replication of Asaia. | We are actually working on insert the others 2 proteins we want to express (p25, p28) on C3 and trying to finally ligate the BioBricks in the BaseVector containing the origin of replication of Asaia. | ||
Revision as of 15:47, 24 October 2010
BioBricks
For the moment we have 14 BioBricks verified by sequencing and by electophoresis. Those BioBricks are in the vector pSB1C3 (friendly called C3) and are ready to be send to the registry!
The BB we have for the moment are:
- C3 + Ori
- C3 + Immu
- C3 + S
- C3 + Ori + Kan
- C3 + Ori + Tet
- C3 + S + CFP
- C3 + S + Immuno
- C3 + S + P25
- C3 + S + immuno + ori + Kan
- C3 + S + P28 + Ori + Tet
- C3 + Ori + Amp
- C3 + W
- C3 + W + Immun
- C3 + W + P25
We are actually working on insert the others 2 proteins we want to express (p25, p28) on C3 and trying to finally ligate the BioBricks in the BaseVector containing the origin of replication of Asaia.
Legend:
- OriA = Origin of replication for Asaia
- PromS = Strong Promoter
- Prom W = Weak Promoter
- RBS = Ribosome Binding Site
- Immu = immunotoxin
Test 2
asd
