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Revision as of 16:50, 21 October 2010 (view source) AndreasConstantinou (Talk | contribs) (→Andreas) ← Older edit		Revision as of 15:32, 24 October 2010 (view source) NinaSchiller (Talk | contribs) (→Gel verification) Newer edit →
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	'''Results'''<br />		'''Results'''<br />
	Incorrect size. Send to the Uppsala team for analysis.		Incorrect size. Send to the Uppsala team for analysis.
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		+	==Nina==
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		+	===Agarose gel on Colony PCR===
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		+	I ran agarose gels on all the colony PCR screens from yesterday to check if I had inserts.
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		+	[[Image:Aq1.jpg|200px]]
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		+	[[Image:Aq2.jpg|200px]]

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Revision as of 15:32, 24 October 2010

Contents 1 Andreas 1.1 Colony PCRs 1.1.1 Gel verification 1.2 PCR for Uppsala team 1.2.1 Gel verification 2 Nina 2.1 Agarose gel on Colony PCR

Andreas

Colony PCRs

• pEX.ProtA⋅His: PA 1 & 2
• pEX.IgGp: Ig 1 & 2

Standard colony PCR settings

• Elongation time: 1:20

Gel verification

Expected bands

• PA: 417 bp
• Ig: 1149 bp

Results
All four constructs confirmed.

PCR for Uppsala team

Gel verification

Expected bands

• All constructs: ≈ 6570 bp

Results
Incorrect size. Send to the Uppsala team for analysis.

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Nina

Agarose gel on Colony PCR

I ran agarose gels on all the colony PCR screens from yesterday to check if I had inserts.