Team:Stockholm/19 October 2010

From 2010.igem.org

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(Andreas)
(Gel verification)
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'''Results'''<br />
'''Results'''<br />
Incorrect size. Send to the Uppsala team for analysis.
Incorrect size. Send to the Uppsala team for analysis.
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==Nina==
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===Agarose gel on Colony PCR===
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I ran agarose gels on all the colony PCR screens from yesterday to check if I had inserts.
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[[Image:Aq1.jpg|200px]]
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[[Image:Aq2.jpg|200px]]

Revision as of 15:32, 24 October 2010


Contents

Andreas

Colony PCRs

  • pEX.ProtA⋅His: PA 1 & 2
  • pEX.IgGp: Ig 1 & 2

Standard colony PCR settings

  • Elongation time: 1:20

Gel verification

Colony PCR of pEX.ProtA*His and pEX.IgGp constructs.
3 μl λ;5 μl sample.
λ = O'GeneRuler 1 kb DNA ladder.

Expected bands

  • PA: 417 bp
  • Ig: 1149 bp

Results
All four constructs confirmed.

PCR for Uppsala team

Gel verification

PCR run for the Uppsala team.
3 μl λ; 5 μl sample
λ = O'GeneRuler 1 kb DNA ladder.

Expected bands

  • All constructs: ≈ 6570 bp

Results
Incorrect size. Send to the Uppsala team for analysis.









Nina

Agarose gel on Colony PCR

I ran agarose gels on all the colony PCR screens from yesterday to check if I had inserts.

Aq1.jpg

Aq2.jpg