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Team:Yale/Our Project/Notebook
From 2010.igem.org
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| - | Extra | + | Extra |
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| - | Wednesday 6/9--First day in lab!--got set up & started cultures | + | Wednesday 6/9--First day in lab!--got set up & started cultures |
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| - | + | Began growing two strains of E. coli--LE392 chosen as a generally healthy option, and DH5alpha chosen because Keasling et. al. used it in their thiosulfate reductase work. | |
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| + | * Spread samples of LE392 (Grindley lab) and DH5alpha (Modis lab) on LB plates and left to incubate overnight at 37 | ||
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Revision as of 14:16, 24 October 2010
our project
lab notebook: week 1 (6/7 - 6/13)
- Monday 6/7--We successfully defend our project to our sponsors(yay!) See more
- Wednesday 6/9--First day in lab!--got set up & started cultures See more
- Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran first copper growth assay in plate reader in PM w/three different ODs of each strain(4 hr. protocol) See more
- Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--b/c allowed to overgrow? See more
