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Team:Groningen/6 September 2010
From 2010.igem.org
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As a follow up experiment for the last [https://2010.igem.org/Team:Groningen/23_August_2010 biofilm induction ] ''Bacillus subtilis'' wit Chaplin E and chaplin H were grown while continuously adding subtilin to keep expression up. After 3hrs of growth they where induced with 1%subtilin. Every hour an additional 0.5%subtilin was added. As a negative control uninduced strains where used. Furthermore shaking cultures (induced and uninduced) where grown simultaneously to check OD levels. Only the chaplin E strain showed reduced growth, but no differences other than retarded growth were visible. | As a follow up experiment for the last [https://2010.igem.org/Team:Groningen/23_August_2010 biofilm induction ] ''Bacillus subtilis'' wit Chaplin E and chaplin H were grown while continuously adding subtilin to keep expression up. After 3hrs of growth they where induced with 1%subtilin. Every hour an additional 0.5%subtilin was added. As a negative control uninduced strains where used. Furthermore shaking cultures (induced and uninduced) where grown simultaneously to check OD levels. Only the chaplin E strain showed reduced growth, but no differences other than retarded growth were visible. | ||
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| + | '''Modellers:''' Work on genexpression model ''Joël, Laura, Djoke'' | ||
Revision as of 12:32, 24 October 2010
Assembly of biobricks in pSB1C3-backbone for submission to parts registry by Maarten
Control restrictions with EcoRI and PstI on constructs:
| 2 µL construct |
| 2 µL buffer O |
| 0,5 µL EcoRI |
| 0,5 µL PstI |
| 15 µL MQ |
| Construct | Expected band sizes (bp) |
|---|---|
| pSB1C3-C | 2031, 848 |
| pSB1C3-DC | 2031, 872 |
| pSB1C3-E | 2031, 317 |
| pSB1C3-H | 2031, 302 |
| pSB1C3-Sortase | 2031, 689 |
| pSB1C3-RFP | 2031, 1069 |
The gel that was run did not show any DNA present on the gel. Experiment was continued on 8 September.
Expression experiments -David & Peter
Two expression experiments were done this week.
Continous induction of chaplins
As a follow up experiment for the last biofilm induction Bacillus subtilis wit Chaplin E and chaplin H were grown while continuously adding subtilin to keep expression up. After 3hrs of growth they where induced with 1%subtilin. Every hour an additional 0.5%subtilin was added. As a negative control uninduced strains where used. Furthermore shaking cultures (induced and uninduced) where grown simultaneously to check OD levels. Only the chaplin E strain showed reduced growth, but no differences other than retarded growth were visible.
Modellers: Work on genexpression model Joël, Laura, Djoke
