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Team:Harvard/parts
From 2010.igem.org
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<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Resistance (Plants)</b></td><td><b>Sequence</b></td></tr> | <tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Resistance (Plants)</b></td><td><b>Sequence</b></td></tr> | ||
<tr><td>V1</td><td>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/37/PORE_O1.gb"> pORE_O1 </a></td></tr> | <tr><td>V1</td><td>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/37/PORE_O1.gb"> pORE_O1 </a></td></tr> | ||
| - | <tr><td>V2</td><td>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td></td></tr> | + | <tr><td>V2</td><td>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fc/PORE_O2.gb"> pORE_O2 </a></td></tr> |
| - | <tr><td>V3</td><td>pORE Expression Series 3 (E3)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>pat</td><td></td></tr> | + | <tr><td>V3</td><td>pORE Expression Series 3 (E3)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/e/e7/PORE_E3.gb"> pORE_E3 </a></td></tr> |
| - | <tr><td>V4</td><td>pORE Expression Series 4 (E4)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>nptII</td><td></td></tr> | + | <tr><td>V4</td><td>pORE Expression Series 4 (E4)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/b/b9/PORE_E4.gb"> pORE_E4 </a></td></tr> |
| - | <tr><td>V5</td><td>pORE Reporter Series 1 (R1)</td><td>Agrobacterium vector with gusA reporter</td><td>Kanamycin</td><td>nptII</td><td></td></tr> | + | <tr><td>V5</td><td>pORE Reporter Series 1 (R1)</td><td>Agrobacterium vector with gusA reporter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fe/PORE_R1.gb"> pORE_R1 </a></td></tr> |
| - | <tr><td>V6</td><td>pORE Reporter Series 3 (R3)</td><td>Agrobacterium vector with smgfp reporter</td><td>Kanamycin</td><td>nptII</td><td></td></tr> | + | <tr><td>V6</td><td>pORE Reporter Series 3 (R3)</td><td>Agrobacterium vector with smgfp reporter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/e/e8/PORE_R3.gb"> pORE_R3 </a></td></tr> |
| - | <tr><td>V7</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr> | + | <tr><td>V7</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7:pORE_O1 biobricked </a></td></tr> |
| - | <tr><td>V8</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr> | + | <tr><td>V8</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8:pORE_O2 biobricked </a></td></tr> |
| - | <tr><td>V9</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr> | + | <tr><td>V9</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9:pORE_E3 biobricked </a></td></tr> |
| - | <tr><td>V10</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr> | + | <tr><td>V10</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10:pORE_E4 biobricked </a></td></tr> |
| - | <tr><td>V11</td><td><center>-</center></td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr> | + | <tr><td>V11</td><td><center>-</center></td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11:pORE_R1 biobricked </a></td></tr> |
| - | <tr><td>V12</td><td><center>-</center></td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr> | + | <tr><td>V12</td><td><center>-</center></td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12:pORE_R3 biobricked </a></td></tr> |
<tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="AmirnaGFP.txt"> amirnaGFP</a></td></tr> | <tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="AmirnaGFP.txt"> amirnaGFP</a></td></tr> | ||
Revision as of 03:33, 24 October 2010
parts and primers
| Name | Original Name | Description | Resistance | Resistance (Plants) | Sequence |
| V1 | pORE Open Series 1 (O1) | Agrobacterium vector open series | Kanamycin | pat | pORE_O1 |
| V2 | pORE Open Series 2 (O2) | Agrobacterium vector open series | Kanamycin | nptII | pORE_O2 |
| V3 | pORE Expression Series 3 (E3) | Agrobacterium vector with ENTCUP2 promoter | Kanamycin | pat | pORE_E3 |
| V4 | pORE Expression Series 4 (E4) | Agrobacterium vector with ENTCUP2 promoter | Kanamycin | nptII | pORE_E4 |
| V5 | pORE Reporter Series 1 (R1) | Agrobacterium vector with gusA reporter | Kanamycin | nptII | pORE_R1 |
| V6 | pORE Reporter Series 3 (R3) | Agrobacterium vector with smgfp reporter | Kanamycin | nptII | pORE_R3 |
| V7 | Agrobacterium vector open series with biobrick cloning site | Kanamycin | pat | V7:pORE_O1 biobricked | |
| V8 | Agrobacterium vector open series with biobrick cloning site | Kanamycin | nptII | V8:pORE_O2 biobricked | |
| V9 | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | pat | V9:pORE_E3 biobricked | |
| V10 | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | nptII | V10:pORE_E4 biobricked | |
| V11 | Agrobacterium vector with gusA reporter and biobrick cloning site | Kanamycin | nptII | V11:pORE_R1 biobricked | |
| V12 | Agrobacterium vector with smgfp reporter and biobrick cloning site | Kanamycin | nptII | V12:pORE_R3 biobricked | |
| A1 | amiRNA GFP | GFP knockdown construct | chloramphenicol | - | amirnaGFP |
| A2 | amiRNA LTP | LTP knockdown construct | chloramphenicol | - | amiRNA LTP |
| A3 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | chloramphenicol | - | ihpRNA Bet1 |
| A4 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | - | ihpRNA LTP |
| A5 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | - | ihpRNA GER |
| F1 | pENTCUP2 promoter | Plant specific promoter. Used to drive expression of our flavor constructs in plants. | chloramphenicol | - | |
| F2 | NOSt terminator | Plant specific terminator. Terminates transcription but no stop codon on end. | chloramphenicol | - | |
| F3 | NOSt terminator + stop | Plant specific terminator. Terminates transcription with stop codon on end. | chloramphenicol | - | |
| F4 | Brazzein | Sweet-tasting protein. 54 amino acid sequence. | chloramphenicol | - | |
| F5 | Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | chloramphenicol | - | |
| F6 | Brazzein YFP2x C-terminus | Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | |
| F7 | Miraculin YFP2x C-terminus | Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | |
| F8 | Brazzein StrepII C-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | |
| F9 | Miraculin StrepII C-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | |
| C1 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown | chloramphenicol | - | |
| C2 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | chloramphenicol | - | |
| C3 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | chloramphenicol | - | |
| GF1 | Barnase | Fatal gene for the genetic fence | chloramphenicol | - | |
| GF2 | Barstar | Protein inhibitor of Barnase | chloramphenicol | - | |
| GF3 | LacIN | Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter | chloramphenicol | - | |
| GF4 | RXRLc-VP16 | Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozide | chloramphenicol | - | |
| GF5 | EcR-Gal4 | Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide | chloramphenicol | - |
