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From 2010.igem.org

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Revision as of 21:04, 23 October 2010

August 10th & 11th

• Grew up Lux Promoters (R0061, R0063, K091146, K145150, K091107, R1062)
• Made SOB media

• Minipreps:

- K091107, K091146, R0063, R1062, K145150
- R0061 did not grow 
- LP(RFP+RBS) + 2 minipreps of RFP+RBS

• Digests:

- Lux promoters with S+P
- RFP+RBS with E+P and X+P(Phosphatased)
- Digested K3+LP with E+P, K3 was phosphatased

• Gel order:

-Ladder
-K091107
-R0063
-R1062
-K091146
-K145150
-RFP+RBS (EP)
-RFP+RBS (XP)
-K3
-LP

• Ligations:

- RFP+RBS + Lux promoters
- K3 + LP
- K3 + RFP+RBS