Team:Michigan/Project
From 2010.igem.org
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== Hy-Bi: Virus Protein Surface Display == | == Hy-Bi: Virus Protein Surface Display == | ||
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+ | The idea is to express algae-binding proteins on the surface of E. Coli in order to cause flocculation. Flocculation is the aggregation and precipitation of particles from solution. This flocculation will make the formation biodiesel more efficient by eliminating the need for centrifugation to concentrate the fuel source. | ||
+ | [[Image:vp130.jpg|370px|right]] | ||
+ | We devised two ways to cause flocculation, one being using a pili expression and the other using a virus surface protein, and the subject of our group is the virus surface protein. During its viral attack on cells, virus needs a protein that enables it to attach to the surface of the host cells. One of the virus surface proteins, vp 130, is used by chlorovirus to attach itself onto the surface of algae. So, if we transfer the gene for this surface binding protein from the virus to E. Coli., each bacteria cell will bind to multiple algae cells, which bind to more bacteria, causing aggregation and flocculation. As opposed to using pili as the means to cause flocculation, the advantage of vp130 is its specific binding and aggregation of algae, automatically yielding a high algae mass in the flocculate. However, there are also disadvantages. For example, vp 130 is not a E. Coli surface protein, so it must be cloned as a fusion to a known surface protein. We also suspected OmpA and ice nucleation protein (INP) as candidate surface proteins. | ||
== Oil Sands: Ag43 Expression == | == Oil Sands: Ag43 Expression == |
Revision as of 17:25, 23 October 2010