Team:Cambridge/Bioluminescence/Bacterial Luciferases
From 2010.igem.org
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==Pathway== | ==Pathway== | ||
Bacterial lux operons emit light using five enzymes: | Bacterial lux operons emit light using five enzymes: | ||
- | *<i>luxA</i> and <i>luxB</i> form the luciferase | + | *<i>luxA</i> and <i>luxB</i> form the luciferase of the system, they emit light using the substrate <b>tetradecanal</b> |
*<i>luxC</i>, <i>luxD</i> and <i>luxE</i> are involved in the biosynthesis of tetradecanal from readily available substrates | *<i>luxC</i>, <i>luxD</i> and <i>luxE</i> are involved in the biosynthesis of tetradecanal from readily available substrates | ||
+ | |||
==Bioluminescent species== | ==Bioluminescent species== | ||
The Cambridge team is looking into these bacterial luciferases: | The Cambridge team is looking into these bacterial luciferases: |
Revision as of 16:31, 23 October 2010
Project Vibrio: Introduction
Project Vibrio was designed to complement Project Firefly, by using bacterial lux operons to do what was impossible with firefly luciferases
- Substrate production in E. coli, avoiding the need to add luciferin
- Emission of blue light
Pathway
Bacterial lux operons emit light using five enzymes:
- luxA and luxB form the luciferase of the system, they emit light using the substrate tetradecanal
- luxC, luxD and luxE are involved in the biosynthesis of tetradecanal from readily available substrates
Bioluminescent species
The Cambridge team is looking into these bacterial luciferases:
- Vibrio fischeri forms symbioses with squid to prevent the squid from casting a shadow on moonlit nights. Due to this their lux proteins are non-functional above 30 degrees
- Vibrio (formerly Photobacterium) phosphoreum emits a very blue light, perhaps partly through its use of [http://partsregistry.org/Part:BBa_K216007 lumazine]