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Team:Groningen/12 July 2010
From 2010.igem.org
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(New page: {{Team:Groningen/Header}} '''Week 28''' {{Team:Groningen/Footer}}) |
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{{Team:Groningen/Header}} | {{Team:Groningen/Header}} | ||
| - | '''Week 28''' | + | '''Week 28, Arend Jan''' |
| + | A mix of the ChpE and ChpH genes were ligated into the pNZ8901-bbs backbone and a restriction check was performed on 8 clones. ChpE contains a DpnI site whereas ChpH does not. A restriction with DpnI and XhoI should yield a 487bp and 176bp fragment for ChpE and just a 648bp fragment for ChpH (+ a ~3500bp backbone fragment for both) | ||
| + | <pre>- 10ul plasmid | ||
| + | - 1.5ul buffer R | ||
| + | - 0.5ul DpnI | ||
| + | - 0.5ul XhoI | ||
| + | - 2.5ul MQ | ||
| + | |||
| + | |||
| + | [[Image:13-07-10gn.jpg|200px|thumb|left|]] | ||
| + | <br style="clear: both" /> | ||
| + | |||
| + | |||
| + | These ligations are performed with the backbone that still contains unwanted IS element with the PstI site. At the same time we are attempting to delete this element using a related backbone. | ||
Revision as of 16:12, 23 October 2010
Hydrophobofilm
pushing coatings into a greener future
Week 28, Arend Jan
A mix of the ChpE and ChpH genes were ligated into the pNZ8901-bbs backbone and a restriction check was performed on 8 clones. ChpE contains a DpnI site whereas ChpH does not. A restriction with DpnI and XhoI should yield a 487bp and 176bp fragment for ChpE and just a 648bp fragment for ChpH (+ a ~3500bp backbone fragment for both)
- 10ul plasmid
- 1.5ul buffer R
- 0.5ul DpnI
- 0.5ul XhoI
- 2.5ul MQ
[[Image:13-07-10gn.jpg|200px|thumb|left|]]
<br style="clear: both" />
These ligations are performed with the backbone that still contains unwanted IS element with the PstI site. At the same time we are attempting to delete this element using a related backbone.
{{Team:Groningen/Footer}}
