Restriction Enzyme Digests

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Revision as of 15:28, 25 June 2010

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http://openwetware.org/wiki/Knight:Restriction_Digest

http://openwetware.org/wiki/Endy:Restriction_Digest

After heat-killing restriction enzymes, add dpni to rid of background plasmids (circular template plasmids that are supposed to be linearized) - dpni works in most buffers

Revision as of 04:08, 25 June 2010 (view source) Cyu286 (Talk \| contribs) ← Older edit		Revision as of 15:28, 25 June 2010 (view source) Cyu286 (Talk \| contribs) (→CHOOOSE) Newer edit →
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	http://openwetware.org/wiki/Endy:Restriction_Digest		http://openwetware.org/wiki/Endy:Restriction_Digest
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		+	After heat-killing restriction enzymes, add dpni to rid of background plasmids (circular template plasmids that are supposed to be linearized) - dpni works in most buffers