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Team:Warsaw/Stage2/Design
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Milena igem (Talk | contribs) (New page: <div class="note"> Design and measurement </div> <p>The safety system we designed and measured consists of the parts specified: <a href="http://partsregistry.org/Part:BBa_I719005">T7 promo...) |
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<div class="note"> Design and measurement </div> | <div class="note"> Design and measurement </div> | ||
| - | <p>The safety system we designed and measured consists of the parts specified: <a href="http://partsregistry.org/Part:BBa_I719005">T7 promoter</a> + <a href="http://partsregistry.org/Part: | + | <p>The safety system we designed and measured consists of the parts specified: <a href="http://partsregistry.org/Part:BBa_I719005">T7 promoter</a> + <a href="http://partsregistry.org/Part:Ba_K299806">MinC</a> + <a href="http://partsregistry.org/Part:BBa_B0032>RBS B0032</a> or <a href="http://partsregistry.org/Part:BBa_B0034">RBS B0034</a> on pSB plasmid, studied in E.coli BL21 strain. </p> |
</html><partinfo>BBa_K299807 DeepComponents</partinfo><html> | </html><partinfo>BBa_K299807 DeepComponents</partinfo><html> | ||
</html><partinfo>BBa_K299808 DeepComponents</partinfo><html> | </html><partinfo>BBa_K299808 DeepComponents</partinfo><html> | ||
<p>We measured our construct in vivo, in the expression system, using strong T7 promoter, IPTG induced. The functionality of our safety element describes optical density of E.coli (OD) and the number of colony forming units/ ml (cfu/ml) in IPTG-induced innoculates BL21 carrying our construct. </p> | <p>We measured our construct in vivo, in the expression system, using strong T7 promoter, IPTG induced. The functionality of our safety element describes optical density of E.coli (OD) and the number of colony forming units/ ml (cfu/ml) in IPTG-induced innoculates BL21 carrying our construct. </p> | ||
Revision as of 12:23, 23 October 2010
The safety system we designed and measured consists of the parts specified: <a href="http://partsregistry.org/Part:BBa_I719005">T7 promoter</a> + <a href="http://partsregistry.org/Part:Ba_K299806">MinC</a> + <a href="http://partsregistry.org/Part:BBa_B0032>RBS B0032</a> or <a href="http://partsregistry.org/Part:BBa_B0034">RBS B0034</a> on pSB plasmid, studied in E.coli BL21 strain.
</html><partinfo>BBa_K299807 DeepComponents</partinfo> <partinfo>BBa_K299808 DeepComponents</partinfo>
We measured our construct in vivo, in the expression system, using strong T7 promoter, IPTG induced. The functionality of our safety element describes optical density of E.coli (OD) and the number of colony forming units/ ml (cfu/ml) in IPTG-induced innoculates BL21 carrying our construct.
