Team:Northwestern/References
From 2010.igem.org
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== Lac Operon Control == | == Lac Operon Control == | ||
- | http://openwetware.org/wiki/IPTG | + | Lac Operon induction and IPTG: http://openwetware.org/wiki/IPTG |
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pbad family (only wildtype available) - http://partsregistry.org/PBAD_Promoter_Family | pbad family (only wildtype available) - http://partsregistry.org/PBAD_Promoter_Family | ||
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==Chitin== | ==Chitin== |
Revision as of 00:50, 23 October 2010
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Lac Operon ControlLac Operon induction and IPTG: http://openwetware.org/wiki/IPTG
Ara Operon Control (pbad promoter)http://partsregistry.org/wiki/index.php?title=Part:BBa_R0080 http://partsregistry.org/wiki/index.php/Part:BBa_I0500 arabinose information: http://openwetware.org/wiki/Arabinose pbad info: http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare pbad family (only wildtype available) - http://partsregistry.org/PBAD_Promoter_Family
ChitinAdding Glucosamine to growth medium boosts chitin production in Yeast (S.C.)- http://ec.asm.org/cgi/reprint/2/5/886 Chitin synthesis Pathway (image) - [http://www.mekarn.org/msc2005-07/thesis07/latslrimage002.jpg] (Figure.1. Biosynthesis of chitin in insects. The pathway starts with trehalose, the main hemolymph sugar in most insects, and ends with the chitin polymer. The diagrammatic representation is based on previously published pathways (Kramer and Koga, 1986; Cohen, 2001).) Chitin Precursor (UDP-N-Acetyl-Glucosamine) is used for Endotoxin (Lipid A) production - http://www.jbc.org/content/268/26/19858.long - possible safety issue - "When bacterial cells arelysed by the immune system, fragments of membrane containing lipid A are released into the circulation, causing fever, diarrhea, and possible fatal endotoxic shock (also called septic shock)." - thus spake wikipedia Chitin Uses: http://www.gmp-chitosan.com/en/products-services/chitin.html Chitin Synthesischitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311 CHS3 does polymerization (Chitin-UDP acetyl-glucosaminyl transferase is just another name for CHS3) - http://www.ebi.ac.uk/interpro/IEntry?ac=IPR004835, http://www.uniprot.org/uniprot/P29465 http://tools.neb.com/NEBcutter2/cutshow.php?name=cb3c5ddc- Chitin Staining (Calcofluor)Does not stain E Coli - http://www.sigmaaldrich.com/etc/medialib/docs/Fluka/Datasheet/18909dat.pdf Stains Biofilm Polysaccharides? - http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6V73-3YKKH2J-4S-1&_cdi=5831&_user=965532&_pii=0043135494003399&_orig=search&_coverDate=08%2F31%2F1995&_sk=999709991&view=c&wchp=dGLbVzz-zSkzS&md5=fedcb287ed9c9dafca2dbb4f2bc053a4&ie=/sdarticle.pdf Inhibits Synthase - http://www.ncbi.nlm.nih.gov/pmc/articles/instance/40653/ Excitation/Emission: http://www.olympusmicro.com/primer/techniques/fluorescence/fluorotable2.html Apoptosisregistry: http://partsregistry.org/Cell_death
Control
Live/Dead Staining aka Viability/Cytotoxicity AssayDMAO(Live), EtD-III(Dead)
Keio Collection - KnockOutsWebsite: http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html Ordering: http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp Method: http://www.pnas.org/content/97/12/6640.full.pdf+html Paper: http://www.nature.com/msb/journal/v2/n1/full/msb4100050.html Primer Extensions: http://www.biomedsearch.com/attachments/00/16/73/85/16738554/msb4100050-s4.xls
ChiA(Chitinase) Knockout (may not be necessary)summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837 chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300 ydgg/tqsA (AI2-Transporter) KnockoutKO -> ~7000x biofilm thickness, ~500x biomass - http://jb.asm.org/cgi/content/short/188/2/587 http://ecoli.aist-nara.ac.jp/GB5/info.jsp?id=JW1593 more info: http://www.open-access-biology.com/biofilms/biofilmsch7.pdf BIF (Biological Imaging Facility)List of Equipment: http://www.northwestern.edu/bioimaging/equip.html
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