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Revision as of 09:34, 24 June 2010 (view source) RachelBoyd (Talk | contribs) (New page: '''Monday''' ===Wet Lab techniques practice=== '''Tips''': * Treat everything as a pathogen! * Work around the bunsen burner. * Use a control. * Clean the bench at the end of the day! * ...) ← Older edit		Revision as of 09:37, 24 June 2010 (view source) RachelBoyd (Talk | contribs) Newer edit →
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	Deena's steak plate picture.		Deena's steak plate picture.
-	===	+	===Set up culture for minipreps===
		+
		+	5ml culture with ''E.coli'' from a single colony grown over night.
		+	Tomorrow we will harvest the DNA.

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Revision as of 09:37, 24 June 2010

Monday

Wet Lab techniques practice

Tips:

• Treat everything as a pathogen!
• Work around the bunsen burner.
• Use a control.
• Clean the bench at the end of the day!
• Heat the wire loop from the middle to the tip.
• Keep plates upside down.
• Keep lids off for as short a time as possible.
• Loosen all the tops off the vessels before you start.
• Flame tops of bottles lightly.

Streak plating

Deena's steak plate picture.

Set up culture for minipreps

5ml culture with E.coli from a single colony grown over night. Tomorrow we will harvest the DNA.