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Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test10settembre bis
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==EXPERIMENT DESCRIPTION== | ==EXPERIMENT DESCRIPTION== | ||
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| + | ===Motivation=== | ||
| + | The experiment was performed to measure the 3OC6-HSL concentration due to the synthesis of homoserine-lactone by 3OC6-HSL generators in saturation phase of the cultures growth. | ||
| + | |||
| + | ===Methods=== | ||
| + | According to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #3|Protocol 3]] the experiment was performed in LB on the following signalling parts: | ||
| + | |||
| + | * <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name: I14) | ||
| + | * <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name: I15) | ||
| + | * <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name: I16) | ||
| + | * <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name: I17) | ||
| + | * <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name: I18) | ||
| + | * <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name: I19) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name: I14-4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name: I15-4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name: I16-4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name: I17-4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name: I18-4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name: I19-4C5) | ||
| + | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0031</partinfo> (negative control) | ||
| + | * <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control) | ||
| + | * <partinfo>pSB1A3</partinfo> - <partinfo>BBa_K</partinfo> (negative control) | ||
| + | |||
==RESULTS (?)== | ==RESULTS (?)== | ||
Revision as of 12:13, 22 October 2010
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