Team:UNIPV-Pavia/Calendar/October
From 2010.igem.org
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Transormation (1ul) into E. coli TOP10 of:I75, I77. They were plated on LB+Amp agar plates and let grow ON at +37°C, 220 rpm. | Transormation (1ul) into E. coli TOP10 of:I75, I77. They were plated on LB+Amp agar plates and let grow ON at +37°C, 220 rpm. | ||
+ | |||
+ | <b>Today we start to transfer all parts that will be sent in <partinfo>pSB1C3</partinfo> plasmid.</b> | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 5th|October, 5th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 5th|October, 5th]]</td><td align="left"> | ||
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Transformation (1ul) of I78 into E. coli TOP10. In order to check our E. coli TOP10 competent cells we plated them on LB+Amp and Cm agar plates. | Transformation (1ul) of I78 into E. coli TOP10. In order to check our E. coli TOP10 competent cells we plated them on LB+Amp and Cm agar plates. | ||
- | ON ligation of I79: I60 + INTEIN_C3. | + | ON ligation of I79: I60 + INTEIN_C3. |
+ | |||
+ | <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 6th|October, 6th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 6th|October, 6th]]</td><td align="left"> | ||
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PCR from colony screening for I78. | PCR from colony screening for I78. | ||
+ | |||
+ | <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 7th|October, 7th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 7th|October, 7th]]</td><td align="left"> | ||
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They were plated on LB+Cm34 agar plates (except for I76 that was plated on LB+Amp agar plate) and let grow ON at +37°C. | They were plated on LB+Cm34 agar plates (except for I76 that was plated on LB+Amp agar plate) and let grow ON at +37°C. | ||
+ | |||
+ | <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 8th|October, 8th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 8th|October, 8th]]</td><td align="left"> | ||
- | 104° Bio-Lab - Glycerol stock, miniprep and Nanodrop quantification of I79-2.This DNA was sent to BMR Genomics for sequencing. | + | 104° Bio-Lab - Glycerol stock, miniprep and Nanodrop quantification of I79-2.This DNA was sent to BMR Genomics for sequencing. |
+ | |||
+ | <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 9th|October, 9th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 9th|October, 9th]]</td><td align="left"> | ||
- | 105° Bio-Lab - | + | 105° Bio-Lab -A new part was cloned with these ones, named I80. It is the basic part to build self-inducible promoters. |
- | A new part was cloned with these ones, named I80. It is the basic part to build self-inducible promoters. | + | |
+ | <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 10th|October, 10th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 10th|October, 10th]]</td><td align="left"> |
Revision as of 10:22, 22 October 2010
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