Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test30settembre

From 2010.igem.org

(Difference between revisions)
(PLATE)
(EXPERIMENT DESCRIPTION)
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==EXPERIMENT DESCRIPTION==
==EXPERIMENT DESCRIPTION==
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===Motivation===
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The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.
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 +
===Methods===
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The experiment was performed in M9 according to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #1|Protocol 1]] on the following measurement parts:
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
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* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
 +
* <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7-4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12-4C5)
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==RESULTS (?)==
==RESULTS (?)==

Revision as of 10:02, 22 October 2010

24° TEST

[SEPTEMBER, 30TH]



PLATE


UNIPV Pavia piastra30settembre.png


EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in M9 according to Protocol 1 on the following measurement parts:

  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
  • <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12-4C5)

RESULTS (?)



MEASUREMENTS

Tecan Test