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Team:Panama/19 October 2010
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(New page: PCR of Rh1ab *Table Electrophoresis: Miniprep samples 1-5 in 0.8% agarose and 1x TBE *Photo We observe bands around 5000bp. It could be the linear plasmid DNA Sample 4 was the only on...)
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(New page: PCR of Rh1ab *Table Electrophoresis: Miniprep samples 1-5 in 0.8% agarose and 1x TBE *Photo We observe bands around 5000bp. It could be the linear plasmid DNA Sample 4 was the only on...)
Newer edit →
Revision as of 23:51, 21 October 2010
PCR of Rh1ab
- Table
Electrophoresis: Miniprep samples 1-5 in 0.8% agarose and 1x TBE
- Photo
We observe bands around 5000bp. It could be the linear plasmid DNA
Sample 4 was the only one that didn't present bands
PCR: to amplify RH1ab, samples of plasmid DNA DNAp=2ul Primer 1= 1 ul Primer 2= 1 ul Master Mix= 12.5 ul Water= 8.5 ul Total= 25 ul
We inoculated 30 colonies en 3 ml liquid LB medium + Amp at 37C, overnight with shaking
30 white colonies and one blue colonies as a negative control
