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UTDallas/20 October 2010
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(New page: *Transformed the ligated PyeaR parts *Miniprepped, digested, ran a gel electrophoresis (took only the bottom band (~3000bp), gel purified, dephosphorylated, and then PCR purified GFP *Liga...)
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(New page: *Transformed the ligated PyeaR parts *Miniprepped, digested, ran a gel electrophoresis (took only the bottom band (~3000bp), gel purified, dephosphorylated, and then PCR purified GFP *Liga...)
Newer edit →
Revision as of 23:22, 21 October 2010
- Transformed the ligated PyeaR parts
- Miniprepped, digested, ran a gel electrophoresis (took only the bottom band (~3000bp), gel purified, dephosphorylated, and then PCR purified GFP
- Ligated GFP to PalkB (the alkane promoter)
- Incubated PyeaR+RFP overnight
