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Team:Panama/27 July 2010
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Today we performed the miniprep of the ''E. coli'' transformed with the RBS and Promoter parts. This miniprep was made in triplicate. | Today we performed the miniprep of the ''E. coli'' transformed with the RBS and Promoter parts. This miniprep was made in triplicate. | ||
Revision as of 23:13, 21 October 2010
July 27
Miniprep Today we did the minipreps of the cultures that we grew for 16 hours yesterday
Today we performed the miniprep of the E. coli transformed with the RBS and Promoter parts. This miniprep was made in triplicate.
Electrophoresis After the miniprep we made an electrophoresis in 1% agarose gel. Also, we measured the DNA concentration with a spectrophotometer:
Expected size
RBS=2092 bp
Promoter=2103 bp
Reporter=2800 bp
Spectrometer measurement
[RBS]=30,4 ηg/μl
Digestion After the DNA concentration measurement, we performed the digestion of the RBS and Promoter parts.
Digestion mix for the Promoter:
NEBuffer2=5μl
BSA=0,5 μl
EcoRI=1 μl
SpeI=1 μl
DNA=20 μl
H2O=22,5μl
Digestion mix for the RBS:
NEBuffer2=5μl
BSA=0,5 μl
XbaI=1 μl
PstI=1 μl
DNA=20 μl
H2O=22,5μl
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