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Team:Stockholm/19 October 2010
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==Andreas== | ==Andreas== | ||
| + | ===Colony PCRs=== | ||
| + | *pEX.ProtA⋅His: PA 1 & 2 | ||
| + | *pEX.IgGp: Ig 1 & 2 | ||
| + | |||
| + | Standard colony PCR settings | ||
| + | *Elongation time: 1:20 | ||
| + | |||
| + | ====Gel verification==== | ||
| + | [[image:ColPCR_pEX.ProtA*His_pEX.IgGp_19oct.png|200px|thumb|right|'''Colony PCR of pEX.ProtA*His and pEX.IgGp constructs.'''<br />3 μl λ;5 μl sample.<br />λ = O'GeneRuler 1 kb DNA ladder.]] | ||
| + | |||
| + | '''Expected bands''' | ||
| + | *PA: 417 bp | ||
| + | *Ig: 1149 bp | ||
| + | |||
| + | '''Results'''<br /> | ||
| + | All four constructs confirmed. | ||
| + | |||
| + | ===PCR for Uppsala team=== | ||
| + | |||
| + | ====Gel verification==== | ||
| + | [[image:Uppsala_team_19oct.png|200px|thumb|right|'''PCR run for the Uppsala team.'''<br />3 μl λ; 5 μl sample<br />λ = O'GeneRuler 1 kb DNA ladder.]] | ||
| + | |||
| + | '''Expected bands'''<br /> | ||
| + | *All constructs: ≈ 6570 bp | ||
| + | |||
| + | '''Results'''<br /> | ||
| + | Incorrect size. Send to the Uppsala team for analysis. | ||
Revision as of 16:50, 21 October 2010
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Contents |
Andreas
Colony PCRs
- pEX.ProtA⋅His: PA 1 & 2
- pEX.IgGp: Ig 1 & 2
Standard colony PCR settings
- Elongation time: 1:20
Gel verification
3 μl λ;5 μl sample.
λ = O'GeneRuler 1 kb DNA ladder.
Expected bands
- PA: 417 bp
- Ig: 1149 bp
Results
All four constructs confirmed.
PCR for Uppsala team
Gel verification
3 μl λ; 5 μl sample
λ = O'GeneRuler 1 kb DNA ladder.
Expected bands
- All constructs: ≈ 6570 bp
Results
Incorrect size. Send to the Uppsala team for analysis.
