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Team:Northwestern/Protocol
From 2010.igem.org
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#With a pipette tip, punch a hole through the foil cover into the corresponding well to the Biobrick™-standard part that you want. Make sure you have properly oriented the plate. We recommend that you do not remove the foil cover, as it could lead to cross contamination between the wells. | #With a pipette tip, punch a hole through the foil cover into the corresponding well to the Biobrick™-standard part that you want. Make sure you have properly oriented the plate. We recommend that you do not remove the foil cover, as it could lead to cross contamination between the wells. | ||
#Add 10uL of diH2O (deionized water) | #Add 10uL of diH2O (deionized water) | ||
| - | #Pipette 1 or 2uL of the resuspended DNA | + | #Pipette 1 or 2uL of the resuspended DNA [[Transformation]] into your desired competent cells, plate bacteria with the appropriate antibiotic* and grow overnight. |
#Pick a single colony and inoculate broth (again, with the correct antibiotic) and grow for 18 hours. | #Pick a single colony and inoculate broth (again, with the correct antibiotic) and grow for 18 hours. | ||
#Use the resulting culture to miniprep the DNA AND make your own glycerol stock (for further instruction on making a glycerol see this page). We recommend using the miniprepped DNA to run QC tests, such as restriction digests and sequencing. | #Use the resulting culture to miniprep the DNA AND make your own glycerol stock (for further instruction on making a glycerol see this page). We recommend using the miniprepped DNA to run QC tests, such as restriction digests and sequencing. | ||
Revision as of 17:11, 23 June 2010
| Home | Team | Project | Parts | Notebook | Safety | Calendar | Protocol |
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Protocol
http://ginkgobioworks.com/support/
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OpenWetWare Protocols: http://openwetware.org/wiki/Protocols
Contents |
DNA (well to stock)
- With a pipette tip, punch a hole through the foil cover into the corresponding well to the Biobrick™-standard part that you want. Make sure you have properly oriented the plate. We recommend that you do not remove the foil cover, as it could lead to cross contamination between the wells.
- Add 10uL of diH2O (deionized water)
- Pipette 1 or 2uL of the resuspended DNA Transformation into your desired competent cells, plate bacteria with the appropriate antibiotic* and grow overnight.
- Pick a single colony and inoculate broth (again, with the correct antibiotic) and grow for 18 hours.
- Use the resulting culture to miniprep the DNA AND make your own glycerol stock (for further instruction on making a glycerol see this page). We recommend using the miniprepped DNA to run QC tests, such as restriction digests and sequencing.
