Team:USTC/Project/safe
From 2010.igem.org
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== experimental safety == | == experimental safety == | ||
- | *From Feb.4th to Feb.13th, a collective training is held targeting to the practice of basic experiment. Setting GFP protein expression system as an example, we learn from the program how to construct standard components and then an expression system. | + | *From Feb.4th to Feb.13th, a collective training is held targeting to the practice of basic experiment. Setting GFP protein expression system as an example, we learn from the program how to construct standard components and then an expression system. Also,it`s the time we get to know each other and become a team as a whole. |
**The contents of the training are listed as followed: | **The contents of the training are listed as followed: |
Revision as of 12:09, 21 October 2010
overview
experimental safety
- From Feb.4th to Feb.13th, a collective training is held targeting to the practice of basic experiment. Setting GFP protein expression system as an example, we learn from the program how to construct standard components and then an expression system. Also,it`s the time we get to know each other and become a team as a whole.
- The contents of the training are listed as followed:
- 1. outlines
- 2. E.coli culture (Medium preparation, Inoculation, Aseptic operation)
- 3. plasmid extraction
- 4. PCR
- 5. DNA Electrophoresis
- 6. Double Digest
- 7. reclaim and appraise of the product of enzyme cutting
- 8. Ligation
- 9. preparation of competent cell
- 10. genomic screening of masculine clone
- 11. DNA sequencing
- The contents of the training are listed as followed:
asking the experts
answering key questions
For iGEM 2010 teams are asked to detail how they approached any issues of biological safety associated with their projects. Specifically, teams should consider the following questions:
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