Team:UNIPV-Pavia/Calendar/October/settimana2

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Revision as of 19:09, 20 October 2010

OCTOBER: WEEK 2

October, 11th

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid

Inoculum of MC42-A, MC43-A, MG42-B, MG43-A, MC43_flip-a,b,c, MG43_flip-a,b,c, MG1655.

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October, 12th

Screening PCR was performed on MC42, MG42, MC43, MG43 and MG1655 with the following primers:

-BBa_K300975, BBa_K300976 (P1-P2)

-BBa_K300976, BBa_K300977 (P2-P3)

-BBa_K300977, BBa_K300978 (P3-P4).

The order of the samples were the follow: MC42, MC43, MG42, MG43, Cneg (MG1655), blank. Primers were (from left to right) BBa_K300975-BBa_K300976, BBa_K300976-BBa_K300977, BBa_K300977-BBa_K300978.

Screening PCR was performed on MC43_flip(A,B,C), MG43_flip(A,B,C) and MG1655 with the following primers:

-BBa_K300975, BBa_K300978 (P1-P4)

-BBa_G00100,BBa_G00101 (VF2-VR)

-BBa_K300977, BBa_K300978 (P3-P4).

The order of the samples were the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_K300975-BBa_K300978, BBa_K300977-BBa_K300978.

The order of the samples were the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_G00100-BBa_G00101.

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid

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October, 13th

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid

^top

October, 14th

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid

^top

October, 15th

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid

^top

October, 16th

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid

^top

@@ Line 46: / Line 46: @@
 Screening PCR was performed on MC42, MG42, MC43, MG43 and MG1655 with the following primers:
--BBa_K300975, BBa_K300976
+-BBa_K300975, BBa_K300976 (P1-P2)
--BBa_K300976, BBa_K300977
+-BBa_K300976, BBa_K300977 (P2-P3)
--BBa_K300977, BBa_K300978.
+-BBa_K300977, BBa_K300978 (P3-P4).
 [[Image:UNIPV10_12_10_2010_TOUCH_DOWN_PCR_CREAM.jpg|thumb|300px|center|The order of the samples were the follow: MC42, MC43, MG42, MG43, Cneg (MG1655), blank. Primers were (from left to right) BBa_K300975-BBa_K300976, BBa_K300976-BBa_K300977, BBa_K300977-BBa_K300978.]]
@@ Line 56: / Line 56: @@
 Screening PCR was performed on MC43_flip(A,B,C), MG43_flip(A,B,C) and MG1655 with the following primers:
--BBa_K300975, BBa_K300978
+-BBa_K300975, BBa_K300978 (P1-P4)
--BBa_G00100,BBa_G00101
+-BBa_G00100,BBa_G00101 (VF2-VR)
--BBa_K300977, BBa_K300978.
+-BBa_K300977, BBa_K300978 (P3-P4).
 [[Image:UNIPV1013_10_10_mcflippati(p1p4)_mgflippati(p1p4)_mcflippati(p3p4) _mgflippati(p3p4).jpg|thumb|300px|center|The order of the samples were the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_K300975-BBa_K300978, BBa_K300977-BBa_K300978.]]