Team:UNIPV-Pavia/Calendar/October/settimana2

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Revision as of 19:08, 20 October 2010

OCTOBER: WEEK 2

October, 11th

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid

Inoculum of MC42-A, MC43-A, MG42-B, MG43-A, MC43_flip-a,b,c, MG43_flip-a,b,c, MG1655.

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October, 12th

Screening PCR was performed on MC42, MG42, MC43, MG43 and MG1655 with the following primers:

-BBa_K300975, BBa_K300976

-BBa_K300976, BBa_K300977

-BBa_K300977, BBa_K300978.

The order of the samples were the follow: MC42, MC43, MG42, MG43, Cneg (MG1655), blank. Primers were (from left to right) BBa_K300975-BBa_K300976, BBa_K300976-BBa_K300977, BBa_K300977-BBa_K300978.

Screening PCR was performed on MC43_flip(A,B,C), MG43_flip(A,B,C) and MG1655 with the following primers:

-BBa_K300975, BBa_K300978

-BBa_G00100,BBa_G00101

-BBa_K300977, BBa_K300978.

The order of the samples were the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_K300975-BBa_K300978, BBa_K300977-BBa_K300978.

The order of the samples were the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_G00100-BBa_G00101.

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid

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October, 13th

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid

^top

October, 14th

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid

^top

October, 15th

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid

^top

October, 16th

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid

^top

@@ Line 46: / Line 46: @@
 Screening PCR was performed on MC42, MG42, MC43, MG43 and MG1655 with the following primers:
--BBa_K300976, BBa_K300977
+-BBa_K300975, BBa_K300976
--BBa_K300977, BBa_K300978
+-BBa_K300976, BBa_K300977
--BBa_K300978, BBa_K300979.
+-BBa_K300977, BBa_K300978.
-[[Image:UNIPV10_12_10_2010_TOUCH_DOWN_PCR_CREAM.jpg|thumb|300px|center|The order of the samples were the follow: MC42, MC43, MG42, MG43, Cneg (MG1655), blank. Primers were (from left to right) BBa_K300976-BBa_K300977, BBa_K300977-BBa_K300978, BBa_K300978-BBa_K300979.]]
+[[Image:UNIPV10_12_10_2010_TOUCH_DOWN_PCR_CREAM.jpg|thumb|300px|center|The order of the samples were the follow: MC42, MC43, MG42, MG43, Cneg (MG1655), blank. Primers were (from left to right) BBa_K300975-BBa_K300976, BBa_K300976-BBa_K300977, BBa_K300977-BBa_K300978.]]
 Screening PCR was performed on MC43_flip(A,B,C), MG43_flip(A,B,C) and MG1655 with the following primers:
--BBa_K300976, BBa_K300979
+-BBa_K300975, BBa_K300978
 -BBa_G00100,BBa_G00101
--BBa_K300978, BBa_K300979.
+-BBa_K300977, BBa_K300978.
-[[Image:UNIPV1013_10_10_mcflippati(p1p4)_mgflippati(p1p4)_mcflippati(p3p4) _mgflippati(p3p4).jpg|thumb|300px|center|The order of the samples were the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_K300976-BBa_K300979, BBa_K300978-BBa_K300979.]]
+[[Image:UNIPV1013_10_10_mcflippati(p1p4)_mgflippati(p1p4)_mcflippati(p3p4) _mgflippati(p3p4).jpg|thumb|300px|center|The order of the samples were the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_K300975-BBa_K300978, BBa_K300977-BBa_K300978.]]
 [[Image:UNIPV10_13-10-10_mcflippati(vf2-vr)_mgflippati(vf2-vr)_cneg_blank.jpg|thumb|300px|center|The order of the samples were the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_G00100-BBa_G00101.]]