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Team:Aberdeen Scotland/Curve Fitting
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<p>Based on a graph in a paper by Witherell et al.<sup style="font-size:10px">[14]</sup> which showed the binding curves of the MS2 stem loop we could calculate more accurately the value for n<sub style="font-size:10px">1</sub>. Our two MS2 stem loops (see Fig 1 in <a href="https://2010.igem.org/Team:Aberdeen_Scotland/Equations">Equations</a>) are 19 nucleotides apart, so our binding curve will most closely resemble that of the 8-16 construct, shown in figure 5A (filled squares).</p> | <p>Based on a graph in a paper by Witherell et al.<sup style="font-size:10px">[14]</sup> which showed the binding curves of the MS2 stem loop we could calculate more accurately the value for n<sub style="font-size:10px">1</sub>. Our two MS2 stem loops (see Fig 1 in <a href="https://2010.igem.org/Team:Aberdeen_Scotland/Equations">Equations</a>) are 19 nucleotides apart, so our binding curve will most closely resemble that of the 8-16 construct, shown in figure 5A (filled squares).</p> | ||
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Revision as of 13:48, 20 October 2010
University of Aberdeen - ayeSwitch
iGEM 2010
Curve Fitting to find the Hill Coefficient for the GFP/Bbox-stem Association (n2)
Based on a graph in a paper by Witherell et al.[14] which showed the binding curves of the MS2 stem loop we could calculate more accurately the value for n1. Our two MS2 stem loops (see Fig 1 in Equations) are 19 nucleotides apart, so our binding curve will most closely resemble that of the 8-16 construct, shown in figure 5A (filled squares).
