Korea U Seoul/27 September 2010

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(New page: ===Ligation=== <br> # Ligation of each parts : PyodA-mAAA, PzntA-RFP and pSB1A2 <br> {| style="border: 1px solid black;" |'''Reagent''' |'''Volume (μL)''' |- |10x T4 DNA ligase reaction ...)
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Revision as of 13:34, 20 October 2010

Ligation


  1. Ligation of each parts : PyodA-mAAA, PzntA-RFP and pSB1A2


Reagent Volume (μL)
10x T4 DNA ligase reaction buffer 2
T4 DNA ligase 2
Each of the digests 2 + 2 + 2 = 8
D.W. 8 / total = 20
Incubation at room temperature for 30min

and stop at 80˚C for 20min


  1. Transformation to E. coli DH5α
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