User:HassanForoughiAsl/22 June 2010

From 2010.igem.org

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Studying EuMelanin (EM):
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;EuMelanin (EM):
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;TODO:
 
#Melanin Gene in recombinant E. Coli.
#Melanin Gene in recombinant E. Coli.
#How IPTG effecting EM production.
#How IPTG effecting EM production.
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#Study this article: Pubmed ID: 17040223
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#Study this article: http://www.ncbi.nlm.nih.gov/pubmed/17040223 Lagunas-Muñoz VH et. al. 2006 :
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#:*Suggests that temperature and PH are the main factors effecting melanin production. they reached a 100% increase in melanin production by changing PH and Temp.
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#:*The production of melanin is subjected to the presence of tyrosinase, which is an enzyme to catalyze the oxidation of phenols (for the melanin case this one is tyrosine) source: wikipedia.
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#:*These enzymes have two activities: they use molecular oxygen to catalyse the hydroxylation of l-tyrosine to l-3,4- dihydroxyphenyl alanine (l-dopa) (cresolase activity); and then oxidizes this product to dopachrome (catecholase activity), which then non-enzymatically oxidizes and polymerizes to form melanin (Garcı´a-Borro´n and Solano 2002).
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#:*EuMel production is what they were interested in, EuMel is directly related to induction of MutMelA gene, which could be controlled by concentration of IPTG. They suggest a 0.1 mmol/litr IPTG for mel production.
#;Ask wetlab team for what their PH, Temp, and IPTG is for their bacteria.
#;Ask wetlab team for what their PH, Temp, and IPTG is for their bacteria.
#Possible pathway in EM production in E. Coli.
#Possible pathway in EM production in E. Coli.
#What else is needed to represent the whole as system!?
#What else is needed to represent the whole as system!?

Revision as of 13:12, 22 June 2010

EuMelanin (EM)
  1. Melanin Gene in recombinant E. Coli.
  2. How IPTG effecting EM production.
  3. Study this article: http://www.ncbi.nlm.nih.gov/pubmed/17040223 Lagunas-Muñoz VH et. al. 2006 :
    • Suggests that temperature and PH are the main factors effecting melanin production. they reached a 100% increase in melanin production by changing PH and Temp.
    • The production of melanin is subjected to the presence of tyrosinase, which is an enzyme to catalyze the oxidation of phenols (for the melanin case this one is tyrosine) source: wikipedia.
    • These enzymes have two activities: they use molecular oxygen to catalyse the hydroxylation of l-tyrosine to l-3,4- dihydroxyphenyl alanine (l-dopa) (cresolase activity); and then oxidizes this product to dopachrome (catecholase activity), which then non-enzymatically oxidizes and polymerizes to form melanin (Garcı´a-Borro´n and Solano 2002).
    • EuMel production is what they were interested in, EuMel is directly related to induction of MutMelA gene, which could be controlled by concentration of IPTG. They suggest a 0.1 mmol/litr IPTG for mel production.
    Ask wetlab team for what their PH, Temp, and IPTG is for their bacteria.
  4. Possible pathway in EM production in E. Coli.
  5. What else is needed to represent the whole as system!?