Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14

From 2010.igem.org

(Difference between revisions)
(→11：DNA extraction from gels)
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=2010/10/14=
=2010/10/14=
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===1:Electrophoresis===
===1:Electrophoresis===
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
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<Result><br />
<Result><br />
[[Image:Matsuura_2010-10-14_14hr_29min.JPG]]
[[Image:Matsuura_2010-10-14_14hr_29min.JPG]]
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===2：DNA extraction===
===2：DNA extraction===
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＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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===3：DNA Ligation ===
===3：DNA Ligation ===
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＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
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===4：DNA Digestion===
===4：DNA Digestion===
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＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
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===5:PCR===
===5:PCR===
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10]
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10]
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-9]
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-9]
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
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===6：DNA extraction from gels===
===6：DNA extraction from gels===
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＜Sample＞<br />
＜Sample＞<br />
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
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(After electrophoresis)
(After electrophoresis)
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＜Protocol＞<br />
＜Protocol＞<br />
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
 +
===7：DNA Digestion===
===7：DNA Digestion===
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＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
 +
===8:PCR===
===8:PCR===
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
 +
===9：DNA extraction from gels===
===9：DNA extraction from gels===
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＜Sample＞<br />
＜Sample＞<br />
*9-7
*9-7
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(After electrophoresis)
(After electrophoresis)
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＜Protocol＞<br />
＜Protocol＞<br />
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
 +
===10:Electrophoresis===
===10:Electrophoresis===
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<Result><br />
<Result><br />
[[Image:Matsuura_2010-10-14_22hr_56min.JPG]]
[[Image:Matsuura_2010-10-14_22hr_56min.JPG]]
 +
===11：DNA extraction from gels===
===11：DNA extraction from gels===

Revision as of 12:35, 19 October 2010

Contents

2010/10/14

1:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-14 14hr 29min.JPG


2：DNA extraction

＜Member＞
nito

＜Sample＞

＜Protocol＞
See Protocol 4


3：DNA Ligation

＜Member＞
nito

＜Sample＞

(after digestion)

＜Protocol＞
See Protocol 10


4：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

  • plasmid

・Digest enzyme

  • XbaⅠ
  • SpeⅠ

＜Protocol＞
See Prptpcpl 9


5:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 1


6：DNA extraction from gels

＜Member＞
nito

＜Sample＞

(After electrophoresis)

＜Protocol＞
SeeProtocol 4


7：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • XbaⅠ
  • SpeⅠ

＜Protocol＞
See Prptpcpl 9


8:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 1


9：DNA extraction from gels

＜Member＞
nito

＜Sample＞

  • 9-7

(After electrophoresis)


＜Protocol＞
SeeProtocol 4


10:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-14 22hr 56min.JPG


11：DNA extraction from gels

＜Member＞
nito

＜Sample＞

(After electrophoresis)

＜Protocol＞
SeeProtocol 4

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/10/14"