Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14
From 2010.igem.org
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- | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
- | *1-2-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3] |
- | *10-2-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-3] |
- | *10-2-9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-9] |
- | *7-8-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] |
(after digestion) | (after digestion) | ||
Revision as of 12:31, 19 October 2010
Contents |
2010/10/14
1:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
2:DNA extraction
<Member>
nito
<Sample>
<Protocol>
See Protocol 4
3:DNA Ligation
<Member>
nito
<Sample>
(after digestion)
<Protocol>
See Protocol 10
4:DNA Digestion
<Member>
nito
<Sample, Materials>
・PCR productions
- plasmid
・Digest enzyme
- XbaⅠ
- SpeⅠ
<Protocol>
See Prptpcpl 9
5:PCR
<Member>
nito
<Sample>
- vector
- 4-5
- 1-2-3
- 10-2-3
- 7-8-10
- 10-2-9
<Protocol>
See Protocol 1
6:DNA extraction from gels
<Member>
nito
<Sample>
- 9-7
(After electrophoresis)
<Protocol>
SeeProtocol 4
7:DNA Digestion
<Member>
nito
<Sample, Materials>
・PCR productions
- 1-2
- 1-2-7
- 10-2-7
・Digest enzyme
- AvrⅡ
- NheⅠ
- XbaⅠ
- SpeⅠ
<Protocol>
See Prptpcpl 9
8:PCR
<Member>
nito
<Sample>
- 9
- 9-7
<Protocol>
See Protocol 1
9:DNA extraction from gels
<Member>
nito
<Sample>
- 9-7
(After electrophoresis)
<Protocol>
SeeProtocol 4
10:Electrophoresis
<Member>
nito
<Sample>
- vector
- 9-7
- 4-5
<Protocol>
See Protocol 8
11:DNA extraction from gels
<Member>
nito
<Sample>
- 9-7
- 4-5
(After electrophoresis)
<Protocol>
SeeProtocol 4