Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/11

From 2010.igem.org

(Difference between revisions)
(→PCR)
(→2010/10/11)
Line 1: Line 1:
 +
{{:Team:Tokyo_Metropolitan/Header}}
{{:Team:Tokyo_Metropolitan/Header}}
{{:Team:Tokyo_Metropolitan/Header}}
=2010/10/11=
=2010/10/11=
-
===Electrophoresis===
+
===1:Electrophoresis===
<Member><br />
<Member><br />
Line 16: Line 17:
[[Image:Matsuura 2010-10-11 01hr 51min.JPG]]
[[Image:Matsuura 2010-10-11 01hr 51min.JPG]]
-
===PCR===
+
===2:PCR===
<Member><br />
<Member><br />
nito
nito
Line 28: Line 29:
<Protocol><br />
<Protocol><br />
-
See Protocol2
+
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
-
(https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)
+
-
 
+
*Tube(temperature in annealing)<br />
*Tube(temperature in annealing)<br />
:1.(60.0,50.0℃)
:1.(60.0,50.0℃)
Line 37: Line 36:
:4.(55.0℃)
:4.(55.0℃)
-
===Electrophoresis===
+
===3:Electrophoresis===
-
 
+
<Member><br />
-
<Member>
+
nito
nito
-
 
+
<Sample><br />
-
 
+
-
<Sample>
+
-
 
+
PCR products.
PCR products.
-
:*9H
+
*9H
-
:*9L
+
*9L
-
:*1-2
+
*1-2
-
:*10-2
+
*10-2
-
:*2
+
*2
-
:*vector
+
*vector
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
+
<Result><br />
-
<Protocol>
+
-
 
+
-
See Protocol8
+
-
 
+
-
(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)
+
-
 
+
-
 
+
-
 
+
-
<Result>
+
-
 
+
[[Image:Matsuura_2010-10-12_00hr_16min.JPG]]
[[Image:Matsuura_2010-10-12_00hr_16min.JPG]]

Revision as of 12:19, 19 October 2010



Contents

2010/10/11

1:Electrophoresis

<Member>
nito

<Sample>
veutor+7-8-10

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-11 01hr 51min.JPG

2:PCR

<Member>
nito

<Sample>

  • 9
  • 1+2
  • 10+2
  • 2
  • vector

<Protocol>
See Protocol 1

  • Tube(temperature in annealing)
1.(60.0,50.0℃)
2.(55.0℃)
3.(55.0℃)
4.(55.0℃)

3:Electrophoresis

<Member>
nito

<Sample>
PCR products.

  • 9H
  • 9L
  • 1-2
  • 10-2
  • 2
  • vector

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-12 00hr 16min.JPG

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/10/11"