Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/10
From 2010.igem.org
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<Sample><br /> | <Sample><br /> | ||
- | *1-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
- | *10-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2] |
(after digestion) | (after digestion) | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
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===5:DNA extraction=== | ===5:DNA extraction=== |
Revision as of 12:07, 19 October 2010
Contents |
2010/10/10
1:Electrophoresis
<Member>
nito
<Sample>
PCR products (10/10)
<Protocol>
See Protocol 8
<Result>
File:Image:Matsuura 2010-10-10 21hr 24min.JPG
2:PCR
<Member>
nito
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 1
3:DNA Digestion
<Member>
nito
<Sample, Materials>
・PCR productions
<Protocol>
See Prptpcpl 9
4:DNA Ligation
<Member>
nito
<Sample>
(after digestion)
<Protocol>
See Protocol 10
5:DNA extraction
<Member>
Mariko,mio,nito
<Sample>
PCR products
- 2
- 4
- 8
- 10
- 5
<Protocol>
See Protocol 4
6:Plasmid extraction from E-coli
<Member>
nito
<Sample>
- 12
- 13
- 15
- 16
<Protocol>
See Protocol 6