Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/29

From 2010.igem.org

(Difference between revisions)
(→2:Electrophoresis)
(→3：DNA Ligation)
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＜Sample＞<br />
＜Sample＞<br />
-
*1,2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1,2]
-
*4,5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4,5]
(after digestion)
(after digestion)
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]
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===4:PCR===
===4:PCR===

Revision as of 11:47, 19 October 2010


Contents

2010/09/29

1：DNA extraction

＜Member＞
nito

＜Sample＞
PCR products

  • 1 (9/27)①
  • 1 (9/27)②
  • 2 (9/27)
  • 3 (9/27)
  • 4 (9/27)①
  • 4 (9/27)②
  • 5(9/27)
  • 8 (9/27)
  • 10(9/27)

Digestion products (9/27)

＜Protocol＞
See Protocol 4

2:Electrophoresis

<Member>
nito

<Sample>
PCR products (9/27)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-29 14hr 34min.JPG

3：DNA Ligation

＜Member＞
nito

＜Sample＞

(after digestion)

＜Protocol＞
See Protocol 3

4:PCR

<Member>
mio,mizuki

<Sample>(temperature in annealing:℃)

  • 5 (70)
  • 6 (63)
  • 7-8(70)
  • 9 (58,63,69,70,72)
  • 10 (58)
  • 1-2(72)

<Protocol>
See Protocol 1
But today we used Kod.

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