Team:Caltech/Week 1

Monday

• Discussed procuring items & lab space.
• Prepared for SURF start.

Tuesday

• Created GANTT Chart (below) detailing our work plan for the summer.

GANTT Chart diagramming our planned progress throughout the summer.

• Detailed possible side projects, open to any member as time permits:
  • Agarase
  • pH/Environmental Response
  • Phototaxis
  • Colored plastic
  • Activa (transglutaminase)
• Procured supplies from stock room
• Cleaned out lab space
• Prepared LB-amp media & plates

Wednesday

• Procured remaining antibiotics & sample of XL1 Blue cells
• Prepare orders for Sigma, NEB, and others
  • Sigma: Crosslinking compounds, substrates (linseed oil & soybean oil)
  • NEB: Biobrick assembly kit, Ladders, polymerase
• Prepared list of biobricks to order
  • Addl Lysis bricks
  • Agarase bricks

Thursday

• Made stock solutions for transformation and subsequent culturing
• Transformed XL-1 Blue E. Coli with selected BioBricks
  • J09250, K124017, K124014, R0082, R0015, R0034, pBAD, I13601

Friday

• Checked transformation
• Minipreped transformed bacteria
  • Checked the plasmid concentration in the mini-prep solution
• Made glycerol stocks from the transformed plasmid