Team:DTU-Denmark/Lab protocols

prepare ligation mix with a total volume of 20 μl
- 2 μl 10x Buffer
- Parts to be ligated: vector+insert 1:5, DNA conc. less than 50 ng
- fill up with water
- 1 μl ligase
- control (no insert)
Which vector to use?

@@ Line 148: / Line 148: @@
 </table>
-<table align="center">
+<table cellpadding=10px>
 <tr>
-   <font color="#333333" face="arial" size="2.5">
+<td width="163px" height="100%" valign="top">
-<td width="570 px">
+   <font color="#990000" face="arial" size="3">
-<h2>Introduction</h2>
+<ul type="circle">
-<p align="justify"></p>
+<li><a href="https://2010.igem.org/Team:DTU-Denmark/Basics">Basics</a></li><br>
+<li><a href="https://2010.igem.org/Team:DTU-Denmark/Regulatory_sytems">Regulatory Systems</a></li><br>
+<li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch">The Switch</a></li><br>
+<li><a href="https://2010.igem.org/Team:DTU-Denmark/SPL">Synthetic Promoter Library</a></li><br>
+<li><a href="https://2010.igem.org/Team:DTU-Denmark/Team1">Anti-Repressor group</a></li><br>
+<li ><a href="https://2010.igem.org/Team:DTU-Denmark/Team2">Anti-Terminator group</a></li><br>
+<li ><a href="https://2010.igem.org/Team:DTU-Denmark/BBrick_Characterisation">Characterisation of Biobricks</a></li><br>
+</ul>
+</td>
+<td>
+<h2>Ligations</h2>
+<h3>Preparation:</h3>
+<ol>
+<li>Calculate your ligase concentration by estimation of DNA concentration from your restriction gel.</li>
+<li>Which vector to use?</li>
+</ol>
+<h3>Material:</h3>
+<ol>
+<li>prepare ligation mix with a total volume of 20 μl
+<ul>
+<li>2 μl 10x Buffer</li>
+<li>Parts to be ligated: vector+insert 1:5, DNA conc. less than 50 ng</li>
+<li>fill up with water</li>
+<li>1 μl ligase</li>
+<li>control (no insert)</li>
+</ul>
+</li>
+<li>Which vector to use?</li>
+</ol>
+</td>
+<td width="163px" height="100%" valign="top">
 </td>
 </tr>