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Revision as of 19:36, 17 October 2010 (view source) Langmo (Talk | contribs) (→Details on the red light/ far-red light diodes and the filters) ← Older edit		Revision as of 19:58, 17 October 2010 (view source) Langmo (Talk | contribs) (→Details on the red light/ far-red light diodes and the filters) Newer edit →
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-	|[[Image:diodeSpectra.jpg|thumb|200px|Figure 7: The approximated spectral energy irradiances of the red light (red curve) and.]]	+	|[[Image:diodeSpectra.jpg|thumb|200px|Figure 7: The approximated spectral energy irradiances of the red light (red curve) and far-red light (blue curve) diodes.]]
-	|[[Image:filterAmplification.jpg|thumb|200px|Figure 8: The casted raw channel with a cover slip and the razor plate needed for preparing the channel.]]	+	|[[Image:filterAmplification.jpg|thumb|200px|Figure 8: The amplifications of the red-light (red curve) and far-red light (blue curve) filters.]]
-	|[[Image:photoconversionCrossSections.jpg|thumb|200px|Figure 9: The assembled channel, yet not bonded.]]	+	|[[Image:photoconversionCrossSections.jpg|thumb|200px|Figure 9: The photoconversion cross-sections of PhyB in the Pr (red curve) and Pfr (blue curve) states.]]
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Revision as of 19:58, 17 October 2010

Microscopy

Details on microscope

A microscope with motorized x, y and z control, a motorized shutter and a 60× lens is used with appropriate fluorescence filters for the fluorescence signals. Light-emitting diode arrays are installed as light sources for red light (660 nm) and far-red light (748 nm) pulses.

Details on the Flow Channel

Figure 2: The mold for the channel. Has to be cleaned after casting.	Figure 3: The casted raw channel with a cover slip and the razor plate needed for preparing the channel.	Figure 4: The assembled channel, yet not bonded.
Figure 5: Bonding the channel.	Figure 6: The ready to use channel under the microscope.

Details on the red light/ far-red light diodes and the filters

Figure 7: The approximated spectral energy irradiances of the red light (red curve) and far-red light (blue curve) diodes.

Figure 8: The amplifications of the red-light (red curve) and far-red light (blue curve) filters.

Figure 9: The photoconversion cross-sections of PhyB in the Pr (red curve) and Pfr (blue curve) states.