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Team:Michigan/Project
From 2010.igem.org
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== '''Overall project''' == | == '''Overall project''' == | ||
| - | Our project has 3 different tracks that we will be working on in the lab simultaneously. These 3 tracks are quorum sensing, pili hyperexpression, and surface display. We made the decision to work in several tracks in order to maximize the efficiency of our lab teams. Our goal is to be able to combine all of these parts in the end and form an organism that will be able to effectively flocculate microalgae. | + | Our project has 3 different tracks that we will be working on in the wet-lab simultaneously. These 3 tracks are quorum sensing, pili hyperexpression, and surface display. We made the decision to work in several tracks in order to maximize the efficiency of our lab teams. Our goal is to be able to combine all of these parts in the end and form an organism that will be able to effectively flocculate microalgae. |
=== Quorum Sensing === | === Quorum Sensing === | ||
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=== Pili Hyperexpression === | === Pili Hyperexpression === | ||
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| + | Type 1 pili are proteinaceous adhesins that are found on the surface of E. coli. The pili help E. coli. form biofilms. By overproducing the pili, we hope to increase flocculation. | ||
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| + | There is also pilus adhesin, which is sticky stuff found on the surface of the pili. Another approach is to hyperproduce this adhesin, which will hopefully increase the adhesion to algae. | ||
=== Surface Display === | === Surface Display === | ||
== Results == | == Results == | ||
Revision as of 17:37, 21 June 2010
Overall project
Our project has 3 different tracks that we will be working on in the wet-lab simultaneously. These 3 tracks are quorum sensing, pili hyperexpression, and surface display. We made the decision to work in several tracks in order to maximize the efficiency of our lab teams. Our goal is to be able to combine all of these parts in the end and form an organism that will be able to effectively flocculate microalgae.
Quorum Sensing
Our cells will use quorum sensing to determine when the flocculation will start.
Pili Hyperexpression
Type 1 pili are proteinaceous adhesins that are found on the surface of E. coli. The pili help E. coli. form biofilms. By overproducing the pili, we hope to increase flocculation.
There is also pilus adhesin, which is sticky stuff found on the surface of the pili. Another approach is to hyperproduce this adhesin, which will hopefully increase the adhesion to algae.
