Team:Calgary/18 June 2010

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'''Friday June 18, 2010'''
'''Friday June 18, 2010'''
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[[Image:06.17.2010_B0015+R0040_PCRGel.png‎|thumb|400px|Jeremy's gel electrophoresis of the colonies resulting from the construction of B0015 (double terminators) and R0040 (TetR repressible promoter)]]
Emily:  Today I did a restriction digest of the arabinose promter (I0500) using all combinations of restriction enzymes.  The sequencing of this part came back very strange, so I want to verify the presence of all of the Biobrick cut sites within the part.  Today I also started a colony PCR using the BBK Construction primers of my I0500-B0034 construct that I constructed yesterday.
Emily:  Today I did a restriction digest of the arabinose promter (I0500) using all combinations of restriction enzymes.  The sequencing of this part came back very strange, so I want to verify the presence of all of the Biobrick cut sites within the part.  Today I also started a colony PCR using the BBK Construction primers of my I0500-B0034 construct that I constructed yesterday.
Dev, Jeremy, and Chris:
Dev, Jeremy, and Chris:

Revision as of 15:29, 21 June 2010

Friday June 18, 2010

Jeremy's gel electrophoresis of the colonies resulting from the construction of B0015 (double terminators) and R0040 (TetR repressible promoter)

Emily: Today I did a restriction digest of the arabinose promter (I0500) using all combinations of restriction enzymes. The sequencing of this part came back very strange, so I want to verify the presence of all of the Biobrick cut sites within the part. Today I also started a colony PCR using the BBK Construction primers of my I0500-B0034 construct that I constructed yesterday.

Dev, Jeremy, and Chris: