Team:Northwestern/Project/Chitin Synthesis

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Revision as of 20:45, 16 October 2010

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Chitin Synthase 3 (CHS3) was cloned out of Saccharomyces Cerevisiae (S.C.) cDNA.

CHS3 from S.C. was chosen because the the protein does not require cofactors or activation factors and also because it was determined to be the most active of the Chitin Synthase family.

Chitin Synthase polymerizes N-Acetyl-D-Glucosamine, also known as Chitin, with substrate as UDP-N-Acetyl-D-Glucosamine.

@@ Line 18: / Line 18: @@
 !align="center"|[[Team:Northwestern/SideProject|<span style="color:#000000;">'''Side Project'''</span>]]
 !align="center"|[[Team:Northwestern/Parts|<span style="color:#000000;">'''Parts'''</span>]]
-!align="center"|[[Team:Northwestern/Notebook|<span style="color:#000000;">'''Notebook'''</span>]]
+!align="center"|[[Team:Northwestern/Notebook|<span style="col#000000;">'''Notebook'''</span>]]
 !align="center"|[[Team:Northwestern/Calendar|<span style="color:#000000;">'''Calendar'''</span>]]
 !align="center"|[[Team:Northwestern/Protocol|<span style="color:#000000;">'''Protocol'''</span>]]
@@ Line 28: / Line 28: @@
 |}
-===Background===
+Chitin Synthase 3 (CHS3) was cloned out of Saccharomyces Cerevisiae (S.C.) cDNA.
-===Forward Primer===
+CHS3 from S.C. was chosen because the the protein does not require cofactors or activation factors and also because it was determined to be the most active of the Chitin Synthase family.
-'-GTG ACT CCT TCG TTG CAT AAT AAT ACT AGT AGC GGC CGC TGC AGG AAG AAA C-3'
+Chitin Synthase polymerizes N-Acetyl-D-Glucosamine, also known as Chitin, with substrate as UDP-N-Acetyl-D-Glucosamine.
-===Reverse Primer===
-===Staining: Calcofluor or Rhodamine-conjugated chitin probe===
-Binds to chitin polymers