Preparation of Competent Cells

From 2010.igem.org

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You guys should buy some TOP10 cells (ask Leonard which ones) and then use the openwetware protocol to prep more when they come in.  Make 500mL (or something like that) and store them in the -80.
 
http://openwetware.org/wiki/Preparing_chemically_competent_cells
http://openwetware.org/wiki/Preparing_chemically_competent_cells

Latest revision as of 18:59, 16 October 2010

http://openwetware.org/wiki/Preparing_chemically_competent_cells