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Week7 7/25/10-7/31/10
From 2010.igem.org
(New page: ===7/26/10=== BIF confocal microscopy training Kit to stock of 1-6G and 1-12O Ran a gel of the pMAL PCR products --> very faint bands Used pMAL PCR products as template for another PCR ...)
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Revision as of 18:03, 16 October 2010
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7/26/10
BIF confocal microscopy training
Kit to stock of 1-6G and 1-12O
Ran a gel of the pMAL PCR products --> very faint bands
Used pMAL PCR products as template for another PCR reaction
Ligated LacP1-GFP and transformed using our own TOP10 competent cells
7/27/10
Excessive growth of 1-6G and 1-12O plates --> amp plates are possibly bad
Gel extraction LacP1, GFP, Linear Tet backbone plasmid restriction digest products
Ran a gel of digestion and ligation products; conclusion: linear plasmid PCR products are bad. Also threw out other assortment of degraded DNA.
3A Assembly using 1 ug of starting DNA.
Poured new Tet and Chl plates
7/28/10
Followed Knight's 3A Assembly for LacP1-GFP part --> No growth
7/29/10
3A Assembly using Knight protocol restriction digests --> but replaced linear backbone plasmid with circular plasmid miniprepped from IGEM kit
7/30/10
LACP1 with GFP in CP-C yielded good results
Ligations (CP1, LACP1, LACPI1 with GFP1 in CP-C)
Redid PCR of CHS3 for pMAL insert
