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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/15
From 2010.igem.org
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<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
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===5:Electrophoresis=== | ===5:Electrophoresis=== | ||
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<Result><br /> | <Result><br /> | ||
[[Image:Matsuura_2010-10-15_19hr_19min.JPG]] | [[Image:Matsuura_2010-10-15_19hr_19min.JPG]] | ||
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===6:PCR=== | ===6:PCR=== | ||
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<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
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===7：DNA Digestion=== | ===7：DNA Digestion=== | ||
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＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ] | ||
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===8：DNA Ligation === | ===8：DNA Ligation === | ||
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＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ] | ||
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===9:PCR=== | ===9:PCR=== | ||
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<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
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===10:PCR=== | ===10:PCR=== | ||
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<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
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===11:Electrophoresis=== | ===11:Electrophoresis=== | ||
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<Result><br /> | <Result><br /> | ||
[[Image:Matsuura_2010-10-15_22hr_32min.JPG]] | [[Image:Matsuura_2010-10-15_22hr_32min.JPG]] | ||
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===12:Transformation=== | ===12:Transformation=== | ||
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<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ] | ||
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===13:Electrophoresis=== | ===13:Electrophoresis=== | ||
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<Result><br /> | <Result><br /> | ||
[[Image:Matsuura_2010-10-16_03hr_24min.JPG]] | [[Image:Matsuura_2010-10-16_03hr_24min.JPG]] | ||
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===14:Electrophoresis=== | ===14:Electrophoresis=== | ||
Revision as of 11:52, 16 October 2010
Contents |
2010/10/15
1：DNA extraction
＜Member＞
Hitomi
＜Sample＞
- 4-5
- 9-7
- vector
＜Protocol＞
SeeProtocol 4
2:Electrophoresis
<Member>
Hitomi
<Sample>
- 4-5
- 9-7
<Protocol>
See Protocol 8
<Result>
3：DNA extraction from gels
＜Member＞
Mariko
＜Sample＞
- 4-5
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
4:PCR
<Member>
Hitomi
<Sample>(temperature in annealing:℃)
- vector
- 4-5
- 9-7
<Protocol>
See Protocol 2
5:Electrophoresis
<Member>
nito
<Sample>
- 4-5
- 9-7
- 9
- vector
<Protocol>
See Protocol 8
<Result>
6:PCR
<Member>
mio
<Sample>
- 9
<Protocol>
See Protocol 2
7：DNA Digestion
＜Member＞
Hitomi
＜Sample, Materials＞
- vector
- 9-7
＜Protocol＞
See Protocol 9
8：DNA Ligation
＜Member＞
Hitomi
＜Sample＞
- 1-2-3
- 4-5
- 6-7
- 9-7
＜Protocol＞
See Protocol 3
9:PCR
<Member>
mio
<Sample>(temperature in annealing:℃)
- 9
- 9-7
- 1-2-3
- 4-5
- 6-7
- 9-7
<Protocol>
See Protocol 2
10:PCR
<Member>
nito,mizuki
<Sample>
- 9
- vector
<Protocol>
See Protocol 2
11:Electrophoresis
<Member>
mio
<Sample>
- 9
<Protocol>
See Protocol 8
<Result>
12:Transformation
<Member>
nito
<Sample>
- 7-8-10
- 1-2-7
- 10-2-7
- JM109
<Protocol>
See Protocol 7
13:Electrophoresis
<Member>
nito
<Sample>
- 1-2-3
- 4-5
- 6-7
- 9-7
- 1
<Protocol>
See Protocol 8
<Result>
14:Electrophoresis
<Member>
nito
<Sample>
- 9
- vector
<Protocol>
See Protocol 8
<Result>
