Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/15
From 2010.igem.org
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Revision as of 11:49, 16 October 2010
Contents |
2010/10/15
1:DNA extraction
<Member>
Hitomi
<Sample>
- 4-5
- 9-7
- vector
<Protocol>
SeeProtocol 4
2:Electrophoresis
<Member>
Hitomi
<Sample>
- 4-5
- 9-7
<Protocol>
See Protocol 8
3:DNA extraction from gels
<Member>
Mariko
<Sample>
- 4-5
(After electrophoresis)
<Protocol>
SeeProtocol 4
4:PCR
<Member>
Hitomi
<Sample>(temperature in annealing:℃)
- vector
- 4-5
- 9-7
<Protocol>
See Protocol 2
5:Electrophoresis
<Member>
nito
<Sample>
- 4-5
- 9-7
- 9
- vector
<Protocol>
See Protocol 8
6:PCR
<Member>
mio
<Sample>
- 9
<Protocol>
See Protocol 2
7:DNA Digestion
<Member>
Hitomi
<Sample, Materials>
- vector
- 9-7
<Protocol>
See Protocol 9
8:DNA Ligation
<Member>
Hitomi
<Sample>
- 1-2-3
- 4-5
- 6-7
- 9-7
<Protocol>
See Protocol 3
9:PCR
<Member>
mio
<Sample>(temperature in annealing:℃)
- 9
- 9-7
- 1-2-3
- 4-5
- 6-7
- 9-7
<Protocol>
See Protocol 2
10:PCR
<Member>
nito,mizuki
<Sample>
- 9
- vector
<Protocol>
See Protocol 2
11:Electrophoresis
<Member>
mio
<Sample>
- 9
<Protocol>
See Protocol 8
12:Transformation
<Member>
nito
<Sample>
- 7-8-10
- 1-2-7
- 10-2-7
- JM109
<Protocol>
See Protocol 7
13:Electrophoresis
<Member>
nito
<Sample>
- 1-2-3
- 4-5
- 6-7
- 9-7
- 1
<Protocol>
See Protocol 8
<Result>
[[Image:]]
14:Electrophoresis
<Member>
nito
<Sample>
- 9
- vector
<Protocol>
See Protocol 8