Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/12
From 2010.igem.org
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<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
+ | |||
+ | ===2:Plasmid extraction from E-coli=== | ||
+ | <Member><br /> | ||
+ | mizuki | ||
+ | |||
+ | <Sample><br /> | ||
+ | *Promotor | ||
+ | *RBS | ||
+ | |||
+ | <Protocol><br /> | ||
+ | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ] | ||
+ | |||
+ | ===5:DNA Ligation === | ||
+ | <Member><br /> | ||
+ | mio,mizuki | ||
+ | |||
+ | <Sample><br /> | ||
+ | *1-2 | ||
+ | *4-5 | ||
+ | *6A-7 | ||
+ | *7-8-10 | ||
+ | (after digestion) | ||
+ | |||
+ | <Protocol><br /> | ||
+ | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
+ | |||
+ | ==3:DNA Digestion== | ||
+ | <Member><br /> | ||
+ | |||
+ | |||
+ | |||
+ | <Sample, Materials><br /> | ||
+ | ・PCR productions | ||
+ | *1L(8/25) 8μl | ||
+ | *2L(8/26) 8μl | ||
+ | *4H(8/26) 16μl | ||
+ | *5H(8/26) 16μl | ||
+ | |||
+ | ・Digest enzyme | ||
+ | *AvrⅡ | ||
+ | *NheⅠ | ||
+ | *SpeⅠ | ||
+ | |||
+ | |||
+ | <Protocol><br /> | ||
+ | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ] |
Revision as of 10:19, 16 October 2010
Contents |
2010/10/12
1:DNA extraction
<Member>
Mariko
<Sample>
- 1-2
- 10-2
- 2
<Protocol>
See Protocol 4
2:DNA Digestion
<Member>
Mariko
<Sample, Materials>
・PCR product
- 1-2
・Digest enzyme
- Avr2
<Protocol>
See Prptpcpl 9
3:PCR
<Member>
nito
<Sample>
- 9
- 1-2-3
- 4-5
<Protocol>
See Protocol 2
2:Plasmid extraction from E-coli
<Member>
mizuki
<Sample>
- Promotor
- RBS
<Protocol>
See Protocol 6
5:DNA Ligation
<Member>
mio,mizuki
<Sample>
- 1-2
- 4-5
- 6A-7
- 7-8-10
(after digestion)
<Protocol>
See Protocol 10
3:DNA Digestion
<Member>
<Sample, Materials>
・PCR productions
- 1L(8/25) 8μl
- 2L(8/26) 8μl
- 4H(8/26) 16μl
- 5H(8/26) 16μl
・Digest enzyme
- AvrⅡ
- NheⅠ
- SpeⅠ
<Protocol>
See Protocol 9