Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/12

From 2010.igem.org

(Difference between revisions)
(New page: =2010/10/12= ===1：DNA extraction=== ＜Member＞<br /> Mariko ＜Sample＞<br /> *1-2 *10-2 *2 ＜Protocol＞<br /> See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern...)
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===1：DNA extraction===
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===2：DNA extraction===
＜Member＞<br />
＜Member＞<br />
Mariko
Mariko
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＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
 +
===7：DNA Digestion===
 +
＜Member＞<br />
 +
nito
 +
 +
＜Sample, Materials＞<br />
 +
・PCR productions
 +
*1
 +
*2
 +
*3
 +
*4
 +
*6
 +
*10
 +
 +
・Digest enzyme
 +
*Avr2
 +
*Nhe1
 +
 +
＜Protocol＞<br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
 +
 +
===1:PCR===
 +
<Member><br />
 +
hito
 +
 +
<Sample>(temperature in annealing:℃)<br />
 +
*1
 +
*2
 +
*3
 +
*4
 +
*5
 +
*6
 +
*7
 +
*8
 +
*9
 +
*10
 +
*1-2
 +
*4-5
 +
*7-8-10
 +
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]

Revision as of 10:11, 16 October 2010

Contents

2010/10/12

2：DNA extraction

＜Member＞
Mariko

＜Sample＞

  • 1-2
  • 10-2
  • 2

＜Protocol＞
See Protocol 4

7：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

  • 1
  • 2
  • 3
  • 4
  • 6
  • 10

・Digest enzyme

  • Avr2
  • Nhe1

＜Protocol＞
See Prptpcpl 9

1:PCR

<Member>
hito

<Sample>(temperature in annealing:℃)

  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 8
  • 9
  • 10
  • 1-2
  • 4-5
  • 7-8-10

<Protocol>
See Protocol 2

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/10/12"