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Team:Yale/LabNotebook/Week2
From 2010.igem.org
(New page: Monday 6/14--Primer design, redo of Friday's growth assay Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday ...) |
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| - | Monday 6/14--Primer design, redo of Friday's growth assay | + | '''Monday 6/14'''--Primer design, redo of Friday's growth assay |
| - | Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived | + | '''Tuesday 6/15'''--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived |
| - | Wednesday 6/16--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions | + | '''Wednesday 6/16'''--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions |
| - | Thursday 6/17--more minimal media work and meeting, started BL21 culture | + | '''Thursday 6/17'''--more minimal media work and meeting, started BL21 culture |
| - | Friday 6/18--plasmid pSB74 arrived (!), already in some unspecified E coli, so plated them out | + | '''Friday 6/18'''--plasmid pSB74 arrived (!), already in some unspecified E coli, so plated them out. Transformed promoters (constitutive & IPTG inducible), terminator, and repressor (for inducible promoter) into BL21. |
| - | + | Also ran BL21 copper growth assays for wide and narrow concentrations ranges. | |
| - | Transformed promoters (constitutive & IPTG inducible), terminator, and repressor (for inducible promoter) into BL21. | + | |
| - | |||
| - | |||
| - | Sunday 6/20--in evening inoculate 5 mL liquid cultures | + | '''June the 19th'''--in which it is revealed that there was a transformation-fail |
| + | |||
| + | Redid transformation, this time into LE392, set aside pSB74 containing cells in fridge | ||
| + | |||
| + | '''Sunday 6/20'''--in evening inoculate 5 mL liquid cultures | ||
Revision as of 17:14, 20 June 2010
Monday 6/14--Primer design, redo of Friday's growth assay
Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived
Wednesday 6/16--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions
Thursday 6/17--more minimal media work and meeting, started BL21 culture
Friday 6/18--plasmid pSB74 arrived (!), already in some unspecified E coli, so plated them out. Transformed promoters (constitutive & IPTG inducible), terminator, and repressor (for inducible promoter) into BL21. Also ran BL21 copper growth assays for wide and narrow concentrations ranges.
June the 19th--in which it is revealed that there was a transformation-fail
Redid transformation, this time into LE392, set aside pSB74 containing cells in fridge
Sunday 6/20--in evening inoculate 5 mL liquid cultures
